Sugar Chromatography

Clinical Use:
Background: Although glucose is the commonest reducing substance in urine and faeces, it is not the only sugar of clinical significance that will give a positive reducing substance result. A positive reducing substance from a patient other than a diabetic must be followed up by thin layer chromatography (TLC) for identification of the individual sugars. This is particularly helpful in the preliminary investigation of conditions such as galactosaemia and fructosaemia.
Reference Ranges: A fresh urine or faeces specimen should not normally contain reducing sugars.
Associated Diseases:
Patient Preparation:
Specimen Requirements: Sample type:

  • Samples for sugar chromatography will be identified initially by reducing substance testing.
  • The analysis can be conducted on either urine or faeces.
  • A fresh sample is required, ideally less than 2 hours old.
  • Samples should be taken into plain, white top universal containers.
  • Boric acid urine collections are unsuitable and will be rejected.

Sample identification:

  • Three patient identifiers from
    • Name
    • D.O.B.
    • Address
    • N.H.S. number
    • Unit Number

    should match on the specimen and request form. This check may be performed prior to centrifugation by the Central Sort department.

Turnaround Time: 1 week
Additional Information:
Referred Test: